N-Trimethylsilylimidazole is a derivatisation agent used in mass spectrometry and gas chromatography. It has a wide range of applications. Below, we discuss its uses. This article also addresses Heptafluorobutyrate and Trimethylsilyl ether. It also discusses sugar identification. So, what exactly is N-Trimethylsilimidazole?
Trimethylsilyl ether
1-(Trimethylsilyl)imidazole is a common silylating agent used for alcohols, esters, and 1,3-dicarbonyl compounds. It has a wide range of applications, including derivatization and chiral synthesis of spirotetrahydropyrans. This article provides an overview of the properties of 1-(Trimethylsilyl)imidazole.
The molecule is formed in a two-step process. Aqueous aliquot of sugar is first silylated with TMSI for 2 h at room temperature. After that, it is partitioned with n-hexane and analyzed by GCMS. Finally, the aqueous layer is treated with l-cysteine methyl ester hydrochloride in pyridine.
Heptafluorobutyrate
To derivatize patulin, imines were reacted with Me3SiCF3 via trifluoroacetic acid anhydride. This reaction required the trapping of the intermediate, but gave unisolable products in moderate yields. Bistrifluoromethylated amines were produced in poor yields, which is likely related to the high basicity of the imines.
As a result, the two metabolites formed are similar to each other but have different positions of the oxime group. The derivatizing agent, MSTFA, has a rate constant of k that is equivalent for both derivatives. Heptafluorobutyrate is a better choice than N-Trimethylsilimidazole CAS:18156-74-6 for use in veterinary medicine.
Polyhalogenated acyl derivatives
In addition to trimethylsilyl ethers, the other amines include fumonisins and trichothecenes. The amines have a polar hydroxyl group, and they may be derivatized using organolithium compounds or by silylating them. The result is a polyhalogenated acyl derivative.
These are prepared by the reaction of phenyl trimethylsilane with acetic anhydride and nitric acid. However, the process is not explosive. The amines are reacted in the presence of cop per nitrate/acetic anhydride mixture. In this reaction, the halogenated group is introduced into the phenyl trimethylsilane ring.
Sugar identification
Trimethylsilylimidazole is an effective silylating agent used in quantitative GC analysis of sucrose. It reacts readily with carboxylic acids and hydroxyls. The method is simple and quick, but it is also very prone to crystallization in the presence of high humidity. A derivation step must be undertaken prior to the injection of the silylating agent.
In this study, 17 target compounds from edible and medicinal plants were extracted and analyzed. The sugar levels varied considerably from one part of the plant to another. The concentrations of the sugars were lowest in the leaves, so a sunburst diagram was designed to show the concentration of sugars in the various parts of plants. We used an AutoSystem XL gas chromatograph coupled with a TurboMass mass spectrometer. The carrier gas used in this study was He at a flow rate of 1 mL/min.
